Phylogenetic Analysis of Ryegrass (Lolium rigidum) Populations and the Proliferation of ALS Resistance in Saudi Arabia

Morphological and simple sequence repeat (SSR) approaches were used to determine the genetic diversity of 29 ryegrass (Lolium rigidum) genotypes belonging eight populations collected from several regions in Saudi Arabia. In this study, 50 Silico-developed SSR markers derived genomic expressed tag (EST) microsatellites examined. Analysis variance showed highly significant differences all studied traits. Cluster analysis based on morphological data Lolium using PAST (paleontological statistics) software was performed. According results, clustering mostly genotype location. The sensitive for herbicide clustered one group. addition, EST-SSR markers, we observed existence a considerable number variations among genotypes. From these only 31 produced reasonable amplification products. results that 23 revealed 74.19% polymorphic. alleles detected per primer ranged five LTC SSR1. tested primers amplified 1434 bands across populations, with an average 46.26 primer. polymorphism information content (PIC) values 0.11 0.76 LT EST-SSR5 unweighted pair group method arithmetic (UPGMA) representing essentially their locations herbicide-tolerance levels. Most formed into four clusters, together Moreover, tolerant distinguished ones. relationship between geographical source rigidum Arabia through study. efficiency developed are transferable species. They have been helpful assess population as could be applied differentiate ryegrass.